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Depression: 21st Century Solutions + The Dark Side of Wheat

Abstract Title:

Melatonin regulates angiogenic and inflammatory proteins in MDA-MB-231 cell line and in co-culture with cancer-associated fibroblasts.

Abstract Source:

Anticancer Agents Med Chem. 2016 Apr 21. Epub 2016 Apr 21. PMID: 27102277

Abstract Author(s):

Larissa Bazela Maschio-Signorini, Gabriela Bottaro Gelaleti, Marina Gobbe Moschetta, Thaiz Ferraz Borin, Bruna Victorasso Jardim-Perassi, Juliana Ramos Lopes, Jéssica Zani Lacerda, Rosimeire Aparecida Roela, Newton Antonio Bordin Júnior, Lizandra Akina Corrêa, Jessica Picinin Cardoso, Debora Aparecida Pires de Campos Zuccari

Article Affiliation:

Larissa Bazela Maschio-Signorini

Abstract:

BACKGROUND: Cancer-associated fibroblast (CAFs) are the most abundant cells in the tumor microenvironment, able to secrete growth factors and act on tumor progression. Melatonin is associated with several mechanisms of action with oncostatics and oncoprotectors effects, and also participate in the reduction of synthesis of surrounding fibroblasts and endothelial cells in breast cancer.

OBJECTIVE: The objectives of this study were to determine the effectiveness of melatonin in cell viability and expression of proteins involved in angiogenesis and inflammation in triple-negative mammary tumor cell line (MDA-MB-231) and in co-culture with CAFs.

METHOD: Cell viability was measured by MTT assay and the protein expression was evaluated by Membrane Antibody Array after melatonin treatment.

RESULTS: Melatonin treatment (1 mM) for 48 hours reduced the cell viability of MDA-MB-231, CAFs and co-culture (p<0.05). The semi-quantitative protein analysis showed that when monoculture of tumor cells were compared with co-culture of CAFs, there was a regulation of angiogenic and inflammatory proteins (p<0.05). Melatonin treatment also leads a differential expression of angiogenic and inflammatory proteins in both monoculture and co-culture of tumor cells and CAFs (p<0.05).

CONCLUSION: The influence of CAFs under the tumor microenvironment was confirmed, increasing the malignancy of the tumor. In addition, melatonin is effective in both monoculture and co-culture, regulating angiogenic and inflammatory proteins that contribute to tumor progression. This study show an overview of melatonin ability in regulate angiogenic and inflammatory proteins, and opens the way for exploration of each individual protein in further studies.

Study Type : In Vitro Study

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Sayer Ji
Founder of GreenMedInfo.com

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Depression: 21st Century Solutions + The Dark Side of Wheat

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