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Abstract Title:

Olive (Olea europaea) leaf extract alters the cytokine profile of Leishmania major-infected macrophages: New insight into the underlying mechanism.

Abstract Source:

Parasite Immunol. 2018 Feb 16. Epub 2018 Feb 16. PMID: 29450889

Abstract Author(s):

F Keirandish, N Mosaffa, M J Tarahi, Sh Fallahi

Article Affiliation:

F Keirandish

Abstract:

This study aimed to identify the effects of olive leaf extract (OLE) on IFNγ, TNFα, TGFβ, and nitric oxide (NO) resulted from macrophages infected with Leishmania major (L. major) amastigotes in the culture medium. High-performance liquid chromatography (HPLC) was used to analyze the level of Oleuropein in plant extract. To evaluate the immunomodulatory effects of OLE,the isolated BALB/c mice peritoneal macrophages were infected with L. major promastigotes and treated with 6.25, 12.5, and 25μg/mL concentrations of OLE. To assess the cytokines, supernatants of cell cultures were harvested after 12, 24, and 48 h. Cytokine production was evaluated by ELISA. Nitriteaccumulation in the culture medium was assessed using the Griess reaction. The level of Oleuropein in the extract was 18.45% by HPLC. According to results, the production of IFNγ and TNFα was significantly increased when the infected and/or not infected macrophages with L. major promastigotes were affected by different concentrations of OLE. Conversely, the production of TGFβ was significantly decreased under the same conditions. Furthermore, the colorimetric determination of NO accumulation in the culture medium indicated that OLE has no effect on NO production. The study corroborates theimmunomodulatory effects of OLE on L. major-infected macrophages. This article is protected by copyright. All rights reserved.

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