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Abstract Title:

Apoptotic cell death of human leukaemia U937 cells by ubiquinone-9 purified from Pleurotus eryngii.

Abstract Source:

Nat Prod Res. 2009;23(12):1112-9. PMID: 19662576

Abstract Author(s):

Jeen-Soo Bae, Jin Wook Park, So Hyun Park, Jung Bin Park, Yoon-Hwa Rho, Young Bae Ryu, Kun-Sik Lee, Ki-Hun Park, Young-Seuk Bae

Abstract:

A chloroform extract of the edible mushroom Pleurotus eryngii showed an inhibitory effect on mammalian DNA topoisomerase I. The topoisomerase I inhibitory compound was purified and identified as ubiquinone-9. Ubiquinone-9 was shown to inhibit the activity of topoisomerase I with IC(50) of about 50 microM. Concentration of 110 microM ubiquinone-9 caused 50% growth inhibition of human leukaemia U937 cells, but not that of normal fibroblast NIH3T3 and 3Y1 cells. Ubiquinone-9-induced cell death was characterised with the cleavage of poly (ADP-ribose) polymerase and pro-caspase 3. Furthermore, ubiquinone-9 induced the fragmentation of DNA into an apoptotic DNA ladder, indicating that the inhibitor triggered apoptosis. The induction of apoptosis by ubiquinone-9 was also confirmed using flow cytometry analysis. Taken together, these results suggest that ubiquinone-9 may function by inhibiting oncogenic disease, at least in part, through the inhibition of topoisomerase I activity.

Study Type : In Vitro Study

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Sayer Ji
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